Fluorocitrate inhibition of aconitate hydratase and the tricarboxylate carrier of rat liver mitochondria
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Fluorocitrate inhibition of aconitate hydratase and the tricarboxylate carrier of rat liver mitochondria.
1. The effect of biologically synthesized and purified fluorocitrate on the metabolism of tricarboxylate anions by isolated rat liver mitochondria was investigated, in relation to the claim by Eanes et al. (1972) that this fluoro compound inhibits the tricarboxylate carrier at concentrations at which it has little effect on the aconitate hydratase activity. 2. That the inhibitory action of fluo...
متن کاملThe inhibition by fluorocitrate of rat liver mitochondrial and extramitochondrial aconitate hydratase.
1. The effects of synthetic fluorocitrate were studied on: (a) the oxidation of citrate and cis-aconitate by rat liver mitochondria; (b) the activity of the aconitate hydratase found in the liver cell sap; (c) the activity of the aconitate hydratase solubilized from liver mitochondria. 2. Fluorocitrate was found to be a potent inhibitor of oxidation of citrate but only a weak inhibitor of oxida...
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Fluoroacetate is a potent poison of animals. It is metabolized to fluorocitrate, which is a powerful inhibitor of mammalian aconitate hydratase [aconitase; citrate (isocitrate) hydrolyase] (Peters, 1952). Fluoroacetate and fluoroacetamide, as systemic insecticides for plants, are taken up by the roots or absorbed by the leaves and are then translocated to other parts of the plant. The plants sh...
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The mitochondrial tricarboxylate carrier (TCC) plays an important role in lipogenesis being TCC-responsible for the efflux from the mitochondria to the cytosol of acetyl-CoA, the primer for fatty acid synthesis. In this study, we investigated the effects of two high-fat diets with different fatty acid composition on the hepatic TCC activity. Rats were fed for 3 weeks on a basal diet supplemente...
متن کاملPurification and characterization of the reconstitutively active tricarboxylate transporter from rat liver mitochondria.
The tricarboxylate transporter has been purified in reconstitutively active form from rat liver mitochondria. The transporter was extracted from mitoplasts with Triton X-114 in the presence of cardiolipin and citrate and was then purified by sequential chromatography on hydroxylapatite, Matrex Gel Orange A, Matrex Gel Blue B, and Affi-Gel 501. Analysis of the purified material via sodium dodecy...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1973
ISSN: 0306-3283
DOI: 10.1042/bj1340217